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pbpA [2017-8-11 16:0:3]
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pbpA [2017-8-11 16:0:3]

class B penicillin-binding protein PBP 2A, required for cell wall synthesis during cell elongation
locus
BSU25000
pI
9.57
mw
79.96 kDa
protein length
716 aa Sequence Blast
gene length
2148 bp Sequence Blast
function
formation of a rod-shaped peptidoglycan cell wall, spore outgrowth
product
penicillin-binding protein PBP 2A
essential
no
synonyms
yqgF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,581,771 → 2,583,921

    The protein

    Catalyzed reaction/ biological activity

  • required for [SW|cell wall synthesis] during cell elongation [Pubmed|12896990]
  • driver of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] dynamics [Pubmed|21636744,21636745]

    • Structure

  • [PDB|3VSK] (from Staphylococcus aureus, 45% identity) [pubmed|22846910]

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]
  • localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]

    • Expression and Regulation

    Operons

    genes
    [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]
    description
    [Pubmed|21815947]

    additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
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    Biological materials

    Mutant

  • MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

    • Labs working on this gene/protein

  • [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

    • References

  • 9851991,9139922,12896990,18957862,20487272,21636744,21636745,21815947,23895585,22846910